Asia populated in one migratory swoop

By David Cyranoski

Researchers mapping a massive array of genomes across Asia say they have found evidence that humans covered the continent in a single migratory wave, and share a common ancestry.

The findings were released by the Human Genome Organisation (HUGO) Pan-Asian SNP Consortium which looks at single-nucleotide polymorphisms (SNPs), or variations at individual bases that make up the genetic code. The results challenge the view that Asia was populated by at least two waves of migration.

"In Asia, we are all related," says Edison Liu, a lead author from the Genome Institute of Singapore. "It brings us closer together."

It is thought that a wave of humans emerged from Africa some 60,000-75,000 years ago and travelled along the southern coast of India, into southeast Asia and down to Oceania. But scientists struggled to explain some of the variation seen in Asia today - such as the obvious physical differences between Malaysian and Filipino Negrito populations and other Asians. Some researchers have postulated that a second wave, or series of waves, from a northern route largely repopulated the area, leaving the Negrito and others as relicts of the earlier migration.

The new study, a five-year examination of variation at some 55,000 SNPs in 1928 individuals, found that Negrito populations had a high level of genetic overlap with other southeast Asia populations, suggesting a common ancestry. East Asians, the analysis suggests, share a large degree of common genetic background with southeast Asians but very little with central Asians, seeming to preclude a peopling of east Asia through a northern route via the Eurasian Steppes. And genetic variation within local populations decreased from southeast to northeast Asia. The two observations suggest that diverse peoples living in southeast Asia migrated northwards.

"It's an impressive collection of samples, a huge amount of work and analysis, and it will contribute greatly to the field," says Mark Stoneking, an evolutionary geneticist at Max Planck Institute for Evolutionary Anthropology in Leipzig, Germany, who was not involved in the study.

Asian unity

Merely organizing the work was a massive task. Researchers in 11 countries and regions took samples from 73 populations, requiring countries often at political or economic loggerheads to share ideas, technology and genomes. For countries lacking the technological capabilities to do the genetic analysis but loath to ship genetic samples to another country, Liu established a system by which researchers could bring the samples to host countries and do the studies themselves, in collaboration with their hosts. "The chain of custody was never broken," he says. "It was extraordinarily collegial."

The result is not a complete shock. While this study provides the most detailed analysis of genetic diversity among Asians to date, a 2005 study on mitochondrial DNA came to a similar conclusion2. Martin Richards, at the University of Leeds, UK, is a specialist in genetic variation in southeast Asia who led that study. "By and large, [the new study] is not surprising for fans of mitochondrial DNA, I think, but naturally it is very heart-warming," he says.

The new study also supports mitochondrial DNA evidence that challenges the customary "out of Taiwan" model, in which migration from mainland China through Taiwan led to the settlement of southeast Asia and the Pacific islands. Instead it seems Taiwan may have been largely settled from islands in southeast Asia.

But the results are not conclusive, as the authors admit. Stoneking says he was "very surprised that the Negrito populations were not more genetically distinct", and would like to see other supposed relict populations, such as those in New Guinea and Australia, studied in the same kind of detail. He argues that it is not possible to tell whether extensive genetic intermingling with surrounding populations might have obscured evidence for two waves of migration. He says he has evidence to support the two-wave theory in work yet to be published that looks specifically at mitochondrial DNA and Y-chromosomes of Negrito populations.

Liu says he is discussing plans for a second phase study with much higher resolution - based on 600,000-1 million SNPs. Possible extensions for the new project will be a look at copy number variation (duplications in sections of DNA), a resequencing of mitochondrial DNA and a focus on specific genetic components such as differences between enzymes that metabolize drugs, and human leukocyte antigen variations. It will be especially tantalizing, says Liu, to see if drug-metabolism genes show the same north-south variation in east Asia. "There would be implications for drug response and clinical trials," he says - although he adds that it will not be possible to link specific health information to genotypes across the continent.

Asia populated in one migratory swoop - SciAm
http://www.sciencemag.org/cgi/content/abstract/326/5959/1541
 

HUGO study reveals India as the source of Asian genetic diversity

By: ASHOK B SHARMA

A study done by a consortium of geneticists from 10 Asian countries shows that Indian genetic diversity is the basis of other population in Asia. It says that over 50,000 years ago there was a first single stream entry of humans into India from Africa. From India the human population settled in South-East Asia and from there some of then moved to east and central Asia.

The study tends to refute the age old belief that Aryans as a distinct race who migrated from Central Asia and settled in the plains of north India. If we are believe the origin of humankind in Africa and the first outward stream of humans settling in India and thereafter spreading to other parts of Asia, including Central Asia, then they are same people who might have probably come back and resettled in India from Central Asia However, HUGO has planned to undertake further studies including Central Asia and the Polynesian Islands.

The first ever study of human genomes of Asia conducted by Human Genome Organisation (HUGO) Pan-Asian SNP Consortium also says that some of the Indian population showed evidence of shared ancestry with European population and this is consistent with the expansion of Indo-European speaking population.

This HUGO’s Pan-Asian initiative is a consortium of 90 geneticists and 40 institutions from 10 Asian nations, namely China, India, Indonesia, Japan, Malaysia, Philippines, Singapore, South Korea, Taiwan and Thailand. It collected samples from 1,928 unrelated individuals representing 73 groups of people from 10 countries and 10 linguistic lineages from member countries as well as from two non-Asian population groups of Africo-American and Caucasian ancestry.

HUGO Pan-Asia SNP Consortium study has been recently published the reputed  Science magazine (vol 326) in December 11, 2009 According to the study modern humans evolved in Africa and spread across the world, adapting locally to the selective pressures of climate, food sources and pahogens.

'Tracking genetic variations through human migrations provides clues to evolution of diseases and phenotypes. India can be a crucible for clinical trials of medicines suitable for Asian population,' said Prof Samir Brahmachari, director-general of the apex Indian scientific body, Council of Scientific and Industrial Research (CSIR). Alongwith Prof Brahmachari, Dr Mitali Mukherjee of Institute of Genomics and Integrative Biology  and Dr Partha P Majumdar, head of the human genetics unit of Indian Statistical Institute were represented in the consortium from India.

According to the study the most recent common ancestors of Asians arrived first in India. Later some of them migrated to Thailand and southwards to the land known today as Malaysia, Indonesia and also eastwards to the Philippines. The first group of settlers must have gone very far south before they settled successfully. These includes the Malay Negritos, Philippine Negritos, the East Indonesians and early settlers of the Pacific Islands. Thereafter one or several groups of people migrated North, mixed with previous settlers there and finally formed various population groups we now refer to as Austronesian, Austro-Astiatic, Tai-Kadal, Hmong-Mien and Altaic.

'This study is a milestone not only in the science that emerged, but the consortium that was formed. Ten Asian countries came together in the spirit of solidarity to understand how we related as a people and we finished with a truly Asian scientific community. We overcame shortage of funds and diverse operational constraints through partnerships, good will and cultural sensitivity,' said Prof Edison Liu, executive director Genome Institute of Singapore and President of the HUGO.

The study also reveals that more than 90% of East Asian haplotypes are found in either South-East Asian or Central-South Asian population and shows clinical structure with haplotype diversity decreasing from south to north. Furthermore, 50% of East Asian haplotypes were found in South-East Asia only and 5% were found in Centra-South Asia only, indicating that South-East Asia was a major geographic source of East Asia population.

India has recently achieved a major breakthrough in human genome sequencing. Scientists at CSIR’s affiliate organization, Institute of Genomics and Integrative Biology (IGIB)  sequenced the human genome of an anonymous Indian citizen. The human genome has 3.1 billion basepairs The team at IGIB generated over 51 gigabases of data using next generation sequencing technology, resulting in over 13x coverage of the human genome. This next generation sequencing technology used in this case enables massively parallel sequencing of millions of genomic fragments of 76 base pairs which are then mapped back to the reference genome. This humongous exercise was made possible with the CSIR supercomputing facility at IGIB.

Sequencing of a human genome requires high computational capability and technological know-how in handling sophisticated machines and analyzing huge volume of data. The first human genome sequencing initiative was conceived as early as 1984. In addition to the US, the international human genome project consortium comprised geneticists from UK, France, Germany, Japan and China. This project formally started in 1980 and sequencing was completed in 2003. India then could not be a part of the process due to lack of resources. Currently more than 14 human genomes sequences from different countries have been announced globally. With the completion of its first first human genome sequence, India is now in the league with few select countries like the US, UK, China, Canada and South Korea.

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Indian scientist set to change world history

Poorly written TOI article, but Singhs comments are interesting (I'm assuming the reporter quoted him correctly). 

TNN 5 December 2009, 01:27am IST

VARANASI: Was Max Muller at fault when he propagated the White Invasion theory? Did Aryan Invasion did not take place? Are we going to witness a change in world history? If reports of Prof Lalji Singh — an internationally acclaimed biotechnology expert — are to be believed, recent studies and research work on tracing genetic lineage of primitive human species have already suggested a different world history, enough to prove a major breakthrough in this field.

Prof Singh of Centre for Cellular and Molecular Biology (CCMB), Hyderabad, who was in the city on Friday to inaugurate the three-day international conference on emerging trends in biotechnology organised by School of Biotechnology, Banaras Hindu University, told reporters that the recent studies would also change the theory of White Invasion (given by Max Muller of Germany).

There was no White Invasion, neither there was any Aryan Invasion as was believed in the past, he said referring to the recent studies on diversity of Indian population that had been published in 'Nature' magazine. The studies indicated southern route of migration of East African population (believed to originate 70,000 years ago) towards Indian sub-continent via Gujarat coast and Andaman and Nicobar Islands, he added.

Saying that Indian population was made up of many populations that have varied genetic compositions, he also added recent studies on DNA linkage indicated an invisible thread (trait) that bounded the Indian population comprising populations of other countries in the sub-continent including Pakistan, Sri Lanka and Malaysia, believed to have originated almost 33,000 years ago.

"The study is on to trace the ancestors of Ancestor North Indian (ANI) population, while the ancestors of Ancestor South Indian (ASI) population has been already traced," he said. "Ongee and Jarva species have been established to be the ancestors of ASI population while DNA matching has found resemblance of East African population with Kurumbha species in Kerala and Raghuvanshi of West Bengal," he added.

"We are looking for DNA from Afghanistan, Pakistan and Jammu and Kashmir to trace the origin of ANI population and once that is established, we would be in a position to indicate the movement of ANI population towards European countries that would change the face of world history," he said. "We are also waiting for clearance of Ministry of Environment and Forest to start major project on revival of endangered animal species including Asiatic lion, tiger, leopard and black buck through cloning technique. We are also working for standardisation of cloning process that would prove to be a milestone in conserving endangered animal species," he added.

Prof Lalji Singh is also heading Laboratory for Conservation of Endangered Species (LACONES) for their revival in the country.

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